Akihikari / Koshihikari Doubled-haploid Lines (DHLs) 212 lines These plant materials consist of 212 doubled-haploid lines (DHLs) derived through anther culture of the F1 plants from a cross between two temperate japonica rice varieties, Akihikari and Koshihikari. The genotype for each DHL was determined using 135 RFLP markers and 34 RAPD markers, and a linkage map was constructed. These DHLs have been used in qualitative trait loci (QTL) mapping of cool-temperature tolerance at booting stage. Although the linkage map covered only about 60% of the rice genome due to the lack of polymorphisms, the genotype data for each DHL can be useful for QTL mapping of other complex traits. Please cite the reference below for publication of results using these materials. Genotype data can be accessed at: http://rgp.dna.affrc.go.jp/E/Publicdata.html RGP genetic map: http://rgp.dna.affrc.go.jp/E/publicdata/geneticmap2000/ <Reference>  Takeuchi Y, Hayasaka H, Chiba B, Tanaka I, Shimano T, Yamagishi M, Nagano K, Sasaki S, Yano M. 2001. Mapping quantitative trait loci controlling cool-temperature tolerance at booting stage using doubled-haploid lines of temperate japonica rice cultivars. Breed.Sci. 51:191-197.
		| Parent Variety | N/K BIL98 | N/K CSSL54 | A/K DHL212 | I/K RIL116 | | K/K BIL182 | K/K CSSL39 | | | | K/H//K BIL93 | K/N CSSL44 | | | | S/H BIL85 | S/H CSSL39 | | | | N/K//K BIL127 | N/K//K CSSL41 | | | | N/K//N BIL79 | N/K//N CSSL48 | | | | J/K//K BIL95 | K/IR//K CSSL42 | | | | | K/IR//IR CSSL40 | | |